ELIZABETH EKADASHI

ELIZABETH EKADASHI

Another great Marathi Movie and another masterpiece just after the success of the movie Dr Prakash Baba Amte. 
Shot in the backdrop of Pandharpur makes it even worth watching. Amazing Direction, Cinematography and Screenplay. Hats off to the director Paresh Mokashi for where the film was shot in the most busy times in Pandharpur that is during the yearly Wari of Sant Tukaram and Dyaneshwar.
The USP of the movie is it's simplicity and without a doubt the performance of three main characters played by the kids viz Dyanesh (Shrirang Mahajan), Mukta (Sayali Bhandarkavathekar) and Pushkar Lonarkar. After watching the movie I am still skeptical about how good the kids are in the movie and how do they make acting look so natural.
Watching the movie gives the feel of being in Pandharpur. Each and every location so real and pleasing to  the eyes. Most amazing place in the movie is the place where the main characters live, credits to the research team to find and choose a place like that. Everything in the film so real and fascinating.

Cons - The length of the movie is too short (1 hr 30 mins). By the time the movie engages you the movie is over. And you feel wish it continued.

Anothe Must Watch movie, released on Childrens Day but a movie for every one.

GOLDEN DAYS HAVE INDEED RETURNED TO MARATHI CINEMA.

HPLC for Pharmaceutical Scientists

HPLC FOR PHARMACEUTICAL SCIENTISTS

edited by

Yuri Kazakevich, Rosario Lobrutto

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Handbook of Stability Testing

HANDBOOK OF STABILITY TESTING

edited by

KIM HUYNH-BA

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मैया मोरी मैं नहिं माखन खायो - maiya mori mai nahi makhan khayo

WRITTEN BY - SURDAS

One of the greatest song on relationship between a mother and son sung soulfully by Anup Jalota.


Yashoda maiya and Krishna

Mai nahee makhan khayo, maiyya mori, mori, mori, mori
 Mai nahee makhan khayo
 Re maiyya mori mai nahee makhan khayo
 
 Maiyya mori, bhor bhayo gaiyya ke pachhe
 Tune madhuban mohi pathayo, char prahar bansi bat bhatyo
 Sanjh pade mai ghar aayo
 O (ree maiyya mori, mai kab makhan khayo -3)
 
 Maiyya mori, mai balak bahiya ko chhoto, yeh chhipa kis vidhi payo
 Maiyya yeh gwal bal sab bair pade hain, barbas mukh laptayo
 Re maiyya mori, mai nahee makhan khayo - (2)
 
 Han ree o mori maiya, ari ho meree maiya,
 Ari pyaree meree maiya, ari bholi meree maiya,
 O sundar sundar meree maiya, o pyaree pyaree meree maiya
 Ari ho meree maiya, o kaisee nyari meree maiya
 Maiya too jananee, maiya mori too jananee
 Mann kee aati bholi, too jananee, mann kee aati bholi
 Inake kahe patiayo, ree maiya mori inake kahe patiayo
 Maiya yeh le apni laputi kambaliya, tune bahut hee nach nachayo
 Ni maiya mori mai nahee makhan khayo
 
 Maiya mori, maiya jiya tere kuchh bhed upaj hai
 Tune mohe janyo parayo jayo
 Surdas tab hansi yashoda, le udd kanth lagayo, nain neer bhari aayo
 Kanhaiya kanhaiya tai nahee makhan khayo
 Kanhaiya more tai nahee makhan khayo
 O lalla more tai nahee makhan khayo
 Kanhaiya more tai nahee makhan khayo
 
 Kanhaiya tai nahee makhan khayo, ari maiya mai nahee makhan khayo
 Sun maiya mori, o (sun maiya mori maine hee makhan khayo - (2))
 Re makhan khayo -9, ree maiya mori maine hee makhan khayo
 Sun maiya mori (maine hee makhan -3) khayo

मैया मोरी मैं नहिं माखन खायो |

भोर भयो गैयन के पाछे, मधुवन मोहिं पठायो ।
चार पहर बंसीबट भटक्यो, साँझ परे घर आयो ॥

मैं बालक बहिंयन को छोटो, छींको किहि बिधि पायो ।
ग्वाल बाल सब बैर परे हैं, बरबस मुख लपटायो ॥

तू जननी मन की अति भोरी, इनके कहे पतिआयो ।
यह लै अपनी लकुटि कमरिया, बहुतहिं नाच नचायो ।

जिय तेरे कछु भेद उपजि है, जानि परायो जायो ॥
'सूरदास' तब बिहँसि जसोदा, लै उर कंठ लगायो ॥

HPLC Dwell Volume (Delay Volume)

Explained By
Dr. Gerard Sharp

B.Sc (Hons) in synthetic organic chemistry from Monash University and a PhD in 
Analytical Chemistry from the University of New South Wales.


  The dwell volume (also called the delay volume) is the volume between the mixing location of the solvents and the start of the analytical column. It is not important in isocratic elution because the mobile phase composition is constant.

This volume however is an important consideration when carrying out gradient programming because the mixing ratio you see on the screen is different to the mixing ratio at the start of the analytical column. This is because there is a defined volume between the mixing chamber and the start of the column and depending on the system flow rate, there will be a lag time before the new mobile phase mix moves through this dwell volume and hits the column.

Depending on your system, the mobile phase mixing is either just before (low pressure mixing) or just after (high pressure mixing) the pump. In a low pressure quartenary pump system, the mixing occurs on the low pressure (solvent bottle) side of the pump and is achieved through a proportioning valve arrangement coupled to the mixing chamber. In a high pressure mixing system, there are actually two pumps (binary pump) and the mixing occurs on the high-pressure side of the pumps. The mixing ratio in this case is determined by the speeds of the pistons of the two pumps.
 

In the low pressure case with the quartenary pump, the dwell volume includes:

• the volume of the connecting tubing from the mixer
• the pump itself including check valves
• a damper
• the injection valve
• any in-line filters or guard columns
• all other connecting tubing 

This volume can often be 1-2mL or more thereby introducing a delay time after the mixing chamber. For example, if there is 2.0mL of dwell volume between the mixer and the column and you are pumping at 1.0mL / min, it will take 2.0 minutes for the new mobile phase mix to take effect in the column. The binary pump has less dwell volume because the volume of the pump is eliminated.

IMPORTANCE OF DWELL VOLUME

If the dwell volume is different from system to system as is often the case, retention times will vary. This can lead to confusion when comparing chromatograms run on different systems of the same mixture and is important when transferring methods and comparing retention times to those listed in a standard method. Also, because the time the mixing ratio of the mobile phase hits the column will be different, selectivity may change. Thus peaks might either move apart or move together causing a loss of resolution and chromatograms obtained from two systems will be different.

Also, this topic has received much attention lately with a focus on Fast and Ultra Fast HPLC using UltraHPLC hardware. With many new systems, there is a capability to use small particle size columns with high back pressures and steep gradients to achieve short run times. If analysis times are targeted at only a few minutes, then having a delay time in the same order is unacceptable. By the time the mobile phase ratio has reached the start of the column, the components have already moved through to the detector.

Even in a conventional system there are modifications which can be made to reduce the dwell volume. The length and diameter of the tubing is the first step. By reducing both the internal diameter and the length, the volume is reduced. A consideration with the diameter however is blockages. A standard diameter might be 0.17mm and this can be reduced to 0.12mm however samples and mobile phase has to be carefully filtered to minimise the chance of a blockage occuring. Even so there is still a chance for a line blockage to occur through wear and tear of pump piston seals and rotor seal degradation. The length though can be controlled to ensure there is no excessive connection length between system modules.

Practical HPLC Method Development

PRACTICAL HPLC METHOD DEVELOPMENT

SECOND EDITION

Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch

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स्वतः हिंदुहृदयसम्राट बाळासाहेब ठाकरे यांनी मला पुष्पहार का घातला? - Why Balasaheb Thakrey garlanded me ? - A Tribute to Saheb

स्वतः हिंदुहृदयसम्राट बाळासाहेब ठाकरे यांनी मला पुष्पहार का घातला? - Why Balasaheb Thackrey garlanded me ?

Bala Saheb Thackrey

(1926-2012)

   Followers in India mourned the demise of a great leader Balasaheb Thackrey on 18th of November 2012 , the founder of the party SHIV SENA in 1966. For the love of his more than 20 lakh people gathered in Mumbai on 19th of November 2012 for his final procession to Shivaji Park in Dadar and million others watching on television.

   He was a great leader, orator and cartoonist and known for his hard, fast and firm decision making, but above all these qualities a very good human being and a warm person which many may not be aware seeing his public and on-screen personality, apart from those who met him and knew him personally. Probably the reason why so many personalities from different fields visited him while he was ill and during his final procession and also expressed their gratitude on various social networking site and in the press and media.

     

   May be the neutrals and anti-thackrey people might have been bemused by the attendance of these personalities and common people at the procession, but it was not a surprise for the the common workers of the party called the 'Shiv-Sainiks' as they were aware of the aura of Balasaheb Thackrey.

   This is very uncommon that a party leader sitting at the top in the hierarchy gave access to the very common worker in the party to meet him and to listen to his grievances or just to give blessings. 

   A nucleus with his strong nuclear force kept the nucleons(shiv-sainiks) together in the atom called Shiv sena for so many years.

   A moment with Balasaheb Thackrey that i will always cherish for rest of my life came about 25 years ago when my father visited him. I don't remember that moment as I was too small, but as told by my father, he took me along with him to meet Balasaheb, he and his collegues carried a garland of flowers for Balasaheb with which they greeted him, after which Balasaheb removed the garland and put it around my neck as seen in the picture below.

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The most favourite picture of my life. May be at that moment I did not know who he was but after few years when I saw this picture I felt very proud and lucky.

TRIBUTE TO THE TIGER

Jai Maharashtra !