All HPLC packings are not alike, and no single column can perform a various of desired separations. HPLC packings differ in hydrophobicity, surface coverage, surface area, pore size and particle shape.
Classification of HPLC Columns as per United State pharmacopoeia.
|
L1 |
Octadecyl silane chemically bonded to
porous silica or ceramic microparticles, 1.5 to 10 μm in
diameter, or a monolithic rod. |
|
L2 |
Octadecyl silane chemically bonded to
silica gel of a controlled surface
porosity that has been bonded to a solid spherical core, 30 to 50 μm in diameter |
|
L3 |
Porous silica particles, 5 to 10 μm in
diameter. |
|
L4 |
Silica gel of controlled surface porosity
bonded to a solid spherical core,
30 to 50 μm in diameter |
|
L5 |
Alumina of controlled surface porosity
bonded to a solid spherical core,
30 to 50 μm in diameter. |
|
L6 |
Strong cation-exchange packing:
sulfonated fluorocarbon polymer coated
on a solid spherical core, 30 to 50 μm in diameter. |
|
L7 |
Octyl silane chemically bonded to totally
porous silica particles, 1.5 to 10 μm in diameter. |
|
L8 |
An essentially monomolecular layer of
aminopropyl-silane chemically bonded to totally porous silica gel
support, 3 to 10 μm in diameter |
|
L9 |
Irregular or spherical, totally porous
silica gel having a chemically bonded, strongly acidic
cation-exchange coating, 3 to 10 µm in diameter |
|
L10 |
Nitrile groups chemically bonded to
porous silica particles, 3 to 10 μm in diameter |
|
L11 |
Phenyl groups chemically bonded to porous
silica particles, 1.5 to 10 μm in diameter. |
|
L12 |
Strong anion-exchange packing made by
chemically bonding a quaternary
amine to a solid silica spherical core, 30 to 50 μm in diameter |
|
L13 |
Trimethylsilane chemically bonded to
porous silica particles, 3 to 10 μm in diameter |
|
L14 |
Silica gel having a chemically bonded,
strongly basic quaternary ammonium anion-exchange coating, 5 to 10
µm in diameter. |
|
L15 |
Hexyl silane chemically bonded to totally
porous silica particles, 3 to 10 μm in diameter. |
|
L16 |
Dimethyl silane chemically bonded to
totally porous silica particles, 5 to 10 μm in diameter |
|
L17 |
Strong cation-exchange resin consisting
of sulfonated cross-linked styrene-divinylbenzene copolymer in the
hydrogen form, 7 to 11 μm in diameter |
|
L18 |
Amino and cyano groups chemically bonded
to porous silica particles, 3 to 10 μm in diameter |
|
L19 |
Strong cation-exchange resin consisting
of sulfonated cross-linked styrene-divinylbenzene copolymer in the
calcium form, 9 μm in diameter |
|
L20 |
Dihydroxypropane groups chemically bonded
to porous silica particles,5 to 10 μm in diamete |
|
L21 |
A rigid, spherical styrene-divinylbenzene
copolymer, 5 to 10 μm in diameter. |
|
L22 |
A cation exchange resin made of porous
polystyrene gel with sulfonic acid groups, about 10 μm in size |
|
L23 |
An anion exchange resin made of porous
polymethacrylate or polyacrylate gel with quaternary ammonium
groups, about 10 μm in size. |
|
L24 |
A semi-rigid hydrophilic gel consisting
of vinyl polymers with numerous hydroxyl groups on the matrix surface, 32 to 63 μm in diameter |
|
L25 |
Packing having the capacity to separate
compounds with a MW range from 100 to 5000 daltons (as determined
by polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers. A polymethacrylate resin base,
crosslinked with poly-hydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable. |
|
L26 |
Butyl silane chemically bonded to totally
porous silica particles, 5 to 10 μm in diameter |
|
L27 |
Porous silica particles, 30 to 50 μm in
diameter |
|
L28 |
A multifunctional support, which consists
of a high purity, 100 Å, spherical
silica substrate that has been bonded with anionic (amine) functionality in addition to a conventional reversed phase C8 functionality. |
|
L29 |
Gamma alumina, reversed phase, low carbon
percentage by weight, alumina-based
polybutadiene spherical particles, 5 μm diameter with a pore diameter of 80 Å. |
|
L30 |
Ethyl silane chemically bonded to a
totally porous silica particle, 3 to 10 μm in diameter |
|
L31 |
A strong anion-exchange resin-quaternary
amine bonded on latex particles attached to a core of 8.5 μm
macroporous particles having a pore size of 2000 Å
and consisting of ethylvinylbenzene cross-linked with 55 % divinyl benzene. |
|
L32 |
A chiral ligand-exchange packing-
L-proline copper complex covalently bonded to irregularly shaped
silica particles, 5 to 10 μm in diameter |
|
L33 |
Packing having the capacity to separate
proteins of 4,000 to 400,000 daltons. It is spherical,
silica-based and processed to provide pH stability. |
|
L34 |
Strong cation-exchange resin consisting
of sulfonated cross-linked styrene-divinyl benzene copolymer in
the lead form, about 9 μm in diameter |
|
L35 |
A zirconium-stabilized spherical silica
packing with a hydrophilic (diol-type) molecular monolayer bonded
phase having a pore size of 150 Å. |
|
L36 |
3,5-dinitrobenzoyl derivative of
L-phenylglycine covalently bonded to 5 μm aminopropyl silica |
|
L37 |
Polymethacrylate gel packing having the
capacity to separate proteins by molecular size over a range of
2,000 to 40,000D. |
|
L38 |
Methacrylate-based size-exclusion packing
for water-soluble samples. |
|
L39 |
Hydrophilic polyhydroxymethacrylate gel
of totally porous spherical resin |
|
L40 |
Cellulose
tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 μm to 20 μm in diameter. |
|
L41 |
Immobilized α-acid glycoprotein on
spherical silica particles, 5 μm in diameter. |
|
L42 |
Octylsilane and octadecylsilane groups
chemically bonded to porous silica particles, 5 μm in diameter |
|
L43 |
Pentafluorophenyl groups chemically
bonded to silica particles, 5 to 10 μm in diameter |
|
L44 |
A multifunctional support, which consists
of a high purity, 60 Å, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a conventional reversed phase C8 functionality. |
|
L45 |
Beta cyclodextrin bonded to porous silica
particles, 5 to 10 μm in diameter |
|
L46 |
Polystyrene/divinylbenzene substrate
agglomerated with quaternary amine functionalized latex beads, 10 μm in diameter |
|
L47 |
High capacity anion-exchange microporous
substrate, fully functionalized with a trimethylamine group, 8 μm in diameter. |
|
L48 |
Sulfonated, cross-linked polystyrene with
an outer layer of submicron, porous, anion-exchange microbeads, 15 μm in diameter |
|
L49 |
A reversed-phase packing made by coating
a thin layer of polybutadiene on
to spherical porous zirconia particles, 3 to 10 μm in diameter |
|
L50 |
Multifunction resin with reversed-phase
retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, 3 to 15 μm in diameter, and a surface area of not less than 350 m2/g, substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene. |
|
L51 |
Amylose
tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica
particles, 5 to 10 μm in diameter. |
|
L52 |
A strong cation exchange resin made of
porous silica with sulfopropyl groups, 5 to 10 μm in diameter. |
|
L53 |
Weak cation-exchange resin consisting of
ethylvinylbenzene, 55 % cross-linked with
divinylbenzene copolymer, 3 to 15 μm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 µm in diameter |
|
L54 |
A size exclusion medium made of covalent
bonding of dextran to highly cross-linked porous agarose beads,
about 13 µm in diameter |
|
L55 |
A strong cation exchange resin made of
porous silica coated with polybutadiene-maleic acid copolymer, about 5 µm in diameter. |
|
L56 |
Isopropyl silane chemically bonded to
totally porous silica particles, 3 to 10 μm in diameter. |
|
L57 |
A chiral-recognition protein, ovomucoid,
chemically bonded to silica particles, about 5 μm in diameter,
with a pore size of 120 angstroms. |
|
L58 |
Strong cation-exchange resin consisting
of sulfonated cross-linked styrene-divinylbenzene copolymer in the
sodium form, about 7 to 11 μm diameter |
|
L59 |
To separate proteins by molecular weight
over the range of 10 to 500 kDa. Spherical 10 μm, silica-based,
and processed to provide hydrophilic characteristics
and pH stability |
|
L60 |
Spherical, porous silica gel, 3 to 10 μm
in diameter, surface has been covalently modified with
palmitamidopropyl groups and endcapped. |
|
L61 |
Hydroxide-selective, strong
anion-exchange resin consisting of a highly cross-linked core of 13 μm microporous particles, pore size less than 10 Å, and consisting of ethylvinylbenzene cross-linked with 55 % divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quarternary ammonium ions (6 %). |
|
L62 |
C30 silane bonded phase on a fully porous
spherical silica, 3 to 15 μm in diameter |
