USP Classification of HPLC Columns

All HPLC packings are not alike, and no single column can perform a various of desired separations. HPLC packings differ in hydrophobicity, surface coverage, surface area, pore size and particle shape.


Classification of HPLC Columns as per United State pharmacopoeia.

L1	Octadecyl silane chemically bonded to porous silica or ceramic microparticles, 1.5 to 10 μm in diameter, or a monolithic rod.
L2	Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50 μm in diameter
L3	Porous silica particles, 5 to 10 μm in diameter.
L4	Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 μm in diameter
L5	Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 μm in diameter.
L6	Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 μm in diameter.
L7	Octyl silane chemically bonded to totally porous silica particles, 1.5 to 10 μm in diameter.
L8	An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally porous silica gel support, 3 to 10 μm in diameter
L9	Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter
L10	Nitrile groups chemically bonded to porous silica particles, 3 to 10 μm in diameter
L11	Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter.
L12	Strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50 μm in diameter
L13	Trimethylsilane chemically bonded to porous silica particles, 3 to 10 μm in diameter
L14	Silica gel having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm in diameter.
L15	Hexyl silane chemically bonded to totally porous silica particles, 3 to 10 μm in diameter.
L16	Dimethyl silane chemically bonded to totally porous silica particles, 5 to 10 μm in diameter
L17	Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μm in diameter
L18	Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 μm in diameter
L19	Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 μm in diameter
L20	Dihydroxypropane groups chemically bonded to porous silica particles,5 to 10 μm in diamete
L21	A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 μm in diameter.
L22	A cation exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10 μm in size
L23	An anion exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 10 μm in size.
L24	A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface, 32 to 63 μm in diameter
L25	Packing having the capacity to separate compounds with a MW range from 100 to 5000 daltons (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, crosslinked with poly-hydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable.
L26	Butyl silane chemically bonded to totally porous silica particles, 5 to 10 μm in diameter
L27	Porous silica particles, 30 to 50 μm in diameter
L28	A multifunctional support, which consists of a high purity, 100 Å, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to a conventional reversed phase C8 functionality.
L29	Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5 μm diameter with a pore diameter of 80 Å.
L30	Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10 μm in diameter
L31	A strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5 μm macroporous particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55 % divinyl benzene.
L32	A chiral ligand-exchange packing- L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 μm in diameter
L33	Packing having the capacity to separate proteins of 4,000 to 400,000 daltons. It is spherical, silica-based and processed to provide pH stability.
L34	Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinyl benzene copolymer in the lead form, about 9 μm in diameter
L35	A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150 Å.
L36	3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 μm aminopropyl silica
L37	Polymethacrylate gel packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000D.
L38	Methacrylate-based size-exclusion packing for water-soluble samples.
L39	Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin
L40	Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 μm to 20 μm in diameter.
L41	Immobilized α-acid glycoprotein on spherical silica particles, 5 μm in diameter.
L42	Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 μm in diameter
L43	Pentafluorophenyl groups chemically bonded to silica particles, 5 to 10 μm in diameter
L44	A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a conventional reversed phase C8 functionality.
L45	Beta cyclodextrin bonded to porous silica particles, 5 to 10 μm in diameter
L46	Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, 10 μm in diameter
L47	High capacity anion-exchange microporous substrate, fully functionalized with a trimethylamine group, 8 μm in diameter.
L48	Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 15 μm in diameter
L49	A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 to 10 μm in diameter
L50	Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, 3 to 15 μm in diameter, and a surface area of not less than 350 m2/g, substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene.
L51	Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5 to 10 μm in diameter.
L52	A strong cation exchange resin made of porous silica with sulfopropyl groups, 5 to 10 μm in diameter.
L53	Weak cation-exchange resin consisting of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, 3 to 15 μm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 µm in diameter
L54	A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13 µm in diameter
L55	A strong cation exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5 µm in diameter.
L56	Isopropyl silane chemically bonded to totally porous silica particles, 3 to 10 μm in diameter.
L57	A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 μm in diameter, with a pore size of 120 angstroms.
L58	Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 7 to 11 μm diameter
L59	To separate proteins by molecular weight over the range of 10 to 500 kDa. Spherical 10 μm, silica-based, and processed to provide hydrophilic characteristics and pH stability
L60	Spherical, porous silica gel, 3 to 10 μm in diameter, surface has been covalently modified with palmitamidopropyl groups and endcapped.
L61	Hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13 μm microporous particles, pore size less than 10 Å, and consisting of ethylvinylbenzene cross-linked with 55 % divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quarternary ammonium ions (6 %).
L62	C30 silane bonded phase on a fully porous spherical silica, 3 to 15 μm in diameter